The effect of new potential fungistatic agents on some human blood cells functions

Authors

  • L. E. Kalashnіkova Institute of Bioorganic Chemistry and Petrochemistry of the NAS of Ukraine, Ukraine
  • I. M. Kopernyk Institute of Bioorganic Chemistry and Petrochemistry of the NAS of Ukraine, Ukraine
  • I. V. Semenyuta Institute of Bioorganic Chemistry and Petrochemistry of the NAS of Ukraine, Ukraine
  • L. P. Golod Institute of Bioorganic Chemistry and Petrochemistry of the NAS of Ukraine, Ukraine
  • D. M. Hodyna Institute of Bioorganic Chemistry and Petrochemistry of the NAS of Ukraine, Ukraine
  • L. O. Metelytsya Institute of Bioorganic Chemistry and Petrochemistry of the NAS of Ukraine, Ukraine

DOI:

https://doi.org/10.24959/ophcj.14.786

Keywords:

in vitro screening, oxazoles, nitrogen-containing bisphosphonates, functional biosensors

Abstract

Erythrocytes and neutrophilic leukocytes of human blood have been used as biosensors for in vitro screening of new biologically active functionally substituted oxazoles and nitrogen-containing bisphosphonates with the known fungistatic activity. It has been found that none of the compounds under research do not reveal the hemolytic action in the reaction of osmotic hemolysis of human red blood cells. For all compounds tested the erythrocyte sedimentation rate is changed. The functionally substituted oxazoles decreased the reaction rate approximately by 70%, and nitrogen-containing bisphosphonates increased it approximately by 30%. The reactivity found can indicate high affinity of the compounds studied to erythrocyte membranes adrenoreceptors. It can be used for characteristics and prediction of a number of other potential effects of the antimycotical compounds tested.
According to the NBT-test the functionally substituted oxazoles are activators of the antimicrobial potential of neutrophilic leukocytes and can be considered as potential stimulators of nonspecific human immunity reactivity. The experimental data obtained significantly expand the range of biological effects of the fungistatic compounds synthesized and confirm perspectiveness of their further study as antimycotic agents. In vitro screening with the use of human blood cells as biosensors is an important part of potential drugs research and can be recommended for testing new biologically active compounds with the known biological activity.

Downloads

Download data is not yet available.

References

  1. Martynov A., Farber B., Farber S. Curr. Med. Chem., 2011, Vol. 18, Nо.22, рр.3431-3436.
  2. Okuda T. New York, Springer, 1995, 420 p.
  3. Juha-Pekka Salminen, Ossipov V. Naturforsch., 2002, Vol. 57, рр.248-253.
  4. Haslam E. Cambridge University Press: Cambridge, UK, 1989, 273 p.
  5. Shemchuk L. A., Chernykh V. P., Martynov А. V. Zhurnal organichnoi khimii – Journal of Organic Chemistry, 1997, Vol. 33, No.8, pp.1257-1258.
  6. Masoud M. S., Hagagg S. S., Ali A. E., Nasr N. M. Spectrochim. Acta A Mol. Biomol. Spectrosc., 2012, Vol. 94, рр.256-264.

Downloads

Published

2014-03-06

How to Cite

(1)
Kalashnіkova L. E.; Kopernyk, I. M.; Semenyuta, I. V.; Golod, L. P.; Hodyna, D. M.; Metelytsya, L. O. The Effect of New Potential Fungistatic Agents on Some Human Blood Cells Functions. J. Org. Pharm. Chem. 2014, 12, 79-82.

Issue

Vol. 12 No. 1(45) (2014)

Section

Original Researches

License

Copyright (c) 2014 National University of Pharmacy

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors publishing their works in the Journal of Organic and Pharmaceutical Chemistry agree with the following terms:

1. Authors retain copyright and grant the journal the right of the first publication of the work under Creative Commons Attribution License allowing everyone to distribute and re-use the published material if proper citation of the original publication is given.

2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal’s published version of the work (e.g., post it to an institutional repository or publish it in a book) providing proper citation of the original publication.

3. Authors are permitted and encouraged to post their work online (e.g. in institutional repositories or on authors’ personal websites) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (see The Effect of Open Access).