Determination of catechins in green tea leaves by HPLC compared to spectrophotometry

Authors

DOI:

https://doi.org/10.24959/ophcj.21.238177

Keywords:

green tea, catechins, leaves, high performance liquid chromatography, spectrophotometry

Abstract

Aim. To study the qualitative composition, the quantitative content of catechins in green tea leaves and compare the data obtained with those evaluated by spectrophotometry.
Materials and methods. Green tea leaves used for the analysis were collected in Anhui Province, China. The extract for the HPLC analysis was obtained by the maceration method with 60 % ethanol twice in the raw material/extractant ratio of 1 : 20. In the case of the spectrophotometric analysis, green tea leaves were extracted with 70 % ethanol twice by the maceration method in the raw material/extractant ratio of 1 : 20. The analysis of the extract from green tea leaves was performed by high performance liquid chromatography using a Prominence LC-20 Shimadzu chromatographic system (Japan) with a SPD-20AV spectrophotometric detector, an Agilent Technologies Microsorb-MV-150 column (reversed phase, C18 modified silica gel, length – 150 mm, diameter – 4.6 mm, particles size – 5 μm). Substances in the extract were identified by comparing the retention time and the spectral characteristics of the test substances with the same characteristics of the reference standards. Spectrophotometric measurements were carried out using a UV-1000 single beam spectrophotometer (China) with the pair of S90-309Q quartz square cells.
Results and discussion. Using high performance liquid chromatography 5 catechins were identified.
Among them epigallocatechin-3-O-gallate (10.85 %) predominated, while catechin (0.61 %) had the lowest concentration. The total amount of catechins in green tea leaves was 30.56 and 24.79 % by HPLC and spectrophotometry, respectively. The F- and t-tests showed that there was no significant difference between the results of HPLC and spectrophotometry.
Conclusions. The qualitative composition and the quantitative content of catechins have been determined in the extract from green tea leaves by high performance liquid chromatography and spectrophotometry. Both HPLC and spectrophotometric methods can be used to determine the total catechin content in green tea leaves. The high content of catechins makes the extract promising for further study and creation of new herbal medicinal products and dietary supplements. The results obtained will be used for standardization of green tea leaves and for future pharmacological research of its extract.

Author Biography

Oleksandr Yu. Maslov, National University of Pharmacy of the Ministry of Health of Ukraine

Ph.D. student of department of Analytal Chemistry and Toxilogical Chemistry

References

Seeram, N. P.; Henning, S. M.; Niu, Y.; Lee, R.; Scheuller, H. S.; Heber, D. Catechin and Caffeine Content of Green Tea Dietary Supplements and Correlation with Antioxidant Capacity. J. Agric. Food. Chem. 2006, 54 (5), 1599 – 1603. https://doi.org/10.1021/jf052857r.

Namal Senanayake, S. P. J. Green tea extract: Chemistry, antioxidant properties and food applications – A review. Journal of Functional Foods 2013, 5 (4), 1529 – 1541. https://doi.org/10.1016/j.jff.2013.08.011.

Naldi, M.; Fiori, J.; Gotti, R.; Périat, A.; Veuthey, J.-L.; Guillarme, D.; Andrisano, V. UHPLC determination of catechins for the quality control of green tea. J. Pharm. Biomed. Anal. 2014, 88, 307 – 314. https://doi.org/10.1016/j.jpba.2013.08.054.

Nikoo, M.; Regenstein, J. M.; Ahmadi Gavlighi, H. Antioxidant and Antimicrobial Activities of (-)-Epigallocatechin-3-gallate (EGCG) and its Potential to Preserve the Quality and Safety of Foods. Comprehensive Reviews in Food Science and Food Safety 2018, 17 (3), 732 – 753. https://doi.org/10.1111/1541-4337.12346.

Xingfei, L.; Shunshun, P.; Wenji, Z.; Lingli, S.; Qiuhua, L.; Ruohong, C.; Shili, S. Properties of ACE inhibitory peptide prepared from protein in green tea residue and evaluation of its anti-hypertensive activity. Process Biochem. 2020, 92, 277 – 287. https://doi.org/10.1016/j.procbio.2020.01.021.

Maslov, O. Yu.; Kolisnyk, S. V.; Komisarenko, M. A.; Altukhov, О. О.; Dynnyk, K. V.; Stepanenko, V. I. Study and evaluation antioxidant activity of dietary supplements with green tea extract. Current issues in pharmacy and medicine: science and practice 2021, 14 (2), 215 – 219.

Komes, D.; Horžić, D.; Belščak, A.; Ganić, K. K.; Vulić, I. Green tea preparation and its influence on the content of bioactive compounds. Food Research International 2010, 43 (1), 167 – 176. https://doi.org/10.1016/j.foodres.2009.09.022.

Chu, C.; Deng, J.; Man, Y.; Qu, Y. Green Tea Extracts Epigallocatechin-3-gallate for Different Treatments. BioMed Research International 2017, 2017, 5615647. https://doi.org/10.1155/2017/5615647.

Johnson, R.; Bryant, S.; Huntley, A. L. Green tea and green tea catechin extracts: An overview of the clinical evidence. Maturitas 2012, 73 (4), 280 – 287. https://doi.org/10.1016/j.maturitas.2012.08.008.

Chakrawarti, L.; Agrawal, R.; Dang, S.; Gupta, S.; Gabrani, R. Therapeutic effects of EGCG: a patent review. Expert Opinion on Therapeutic Patents 2016, 26 (8), 907 – 916. https://doi.org/10.1080/13543776.2016.1203419.

Singh, B. N.; Shankar, S.; Srivastava, R. K. Green tea catechin, epigallocatechin-3-gallate (EGCG): Mechanisms, perspectives and clinical applications. Biochem. Pharmacol. 2011, 82 (12), 1807 – 1821. https://doi.org/10.1016/j.bcp.2011.07.093.

Steiniger, D.; Lu, G.; Butler, J.; Phillips, E.; Fintschenko, Y. Determination of Multiresidue Pesticides in Green Tea by Using a Modified QuEChERS Extraction and Ion-Trap Gas Chromatography/Mass Spectrometry. J. AOAC Int. 2019, 93 (4), 1169 – 1179. https://doi.org/10.1093/jaoac/93.4.1169.

Kartsova, L. A.; Ganzha, O. V.; Alekseeva, A. V. Possibilities and limitations of different modes of capillary electrophoresis for the quantitative determination of catechols and caffeine in black and green tea. J. Anal. Chem. 2010, 65 (2), 209 – 214. https://doi.org/10.1134/S1061934810020188.

Maslov, O. Y.; Kolisnyk, S. V.; Kolisnyk, Y. S. Determination the total content of catechins in green tea leaves. Scientific collection «Interconf», Proceeding of the 3rd international scientific and practical conference “Science, education, innovation: topical issues and modern aspects”, Tallin, Estonia, June 25-26, 2021; Ühingu Teadus juhatus: Tallin, 2021; 231 – 233.

Maslov, O. Yu.; Kolisnyk, S. V.; Hrechana, O. V.; Serbin, A. H. Study of the qualitative composition and quantitative content of some groups of BAS in dietary supplements with green tea leaf extract. Zaporozhye Medical Journal 2021, 23 (1), 132 – 137. https://doi.org/10.14739/2310-1210.2021.1.224932.

Maslov, O. Yu.; Kolisnyk, S. V. Vyznachennia yakisnoho skladu katekhiniv v lysti zelenoho chaiu. Tekhnolohichni ta biofarmatsevtychni aspekty stvorennia likarskykh preparativ riznoi napravlenosti dii, Materialy V mizhnarodnoi naukovo-praktychnoi internet konferentsii [Determination of the qualitative composition of catechins in green tea leaves. Technological and biopharmaceutical aspects of creation of medicines of different direction of action, Materials of the V international Internet conference of science and practice], Kharkiv, Ukraine, Nov 26, 2020; NFaU: Kharkiv, 2020 (in Ukrainian).

Shpagina, O. V.; Bondarenko, I. Z.; Kolesnikova, G. S. Statin administration is associated with higher IGF-1 levels in patients without diabetes mellitus. Problems of endocrinology 2018, 64 (4), 200 – 207. https://doi.org/10.14341/probl8759.

Khodakov, I. V. The HPLC Method of Identification of Polyphenols in Plant Extracts by Example of Determination of Isoflavone Composition in Soy Seeds. Methods and objects of chemical analysis 2013, 8 (3), 132 – 142.

Ahmad, R. S.; Butt, M. S.; Huma, N.; Sultan, M. T.; Arshad, M. U.; Mushtaq, Z.; Saeed, F. Quantitative and Qualitative Portrait of Green Tea Catechins (GTC) Through HPLC. International Journal of Food Properties 2014, 17 (7), 1626 – 1636. https://doi.org/10.1080/10942912.2012.723232.

He, Q.; Yao, K.; Jia, D.; Fan, H.; Liao, X.; Shi, B. Determination of total catechins in tea extracts by HPLC and spectrophotometry. Natural Product Research 2009, 23 (1), 93 – 100. https://doi.org/10.1080/14786410801886682.

Downloads

Published

2021-10-20

How to Cite

(1)
Maslov, O. Y.; Komisarenko, M. A.; Kolisnyk, Y. S.; Kostina, T. A. Determination of Catechins in Green Tea Leaves by HPLC Compared to Spectrophotometry. J. Org. Pharm. Chem. 2021, 19, 28-33.

Issue

Section

Original researches