The antioxidant activity of extracts of the mycelium and the culture fluid of medicinal macromycetes of Pholiota (Fr.) P. Kumm. genus

Authors

  • Liubov V. Regeda M.G. Kholodny Institute of Botany of the National Academy of Sciences of Ukraine, Ukraine https://orcid.org/0000-0002-3272-480X
  • Nina A. Bisko M.G. Kholodny Institute of Botany of the National Academy of Sciences of Ukraine, Ukraine https://orcid.org/0000-0003-1894-0896
  • Nina V. Gurinovych M.G. Kholodny Institute of Botany of the National Academy of Sciences of Ukraine, Ukraine

DOI:

https://doi.org/10.24959/ophcj.21.247038

Keywords:

mycology, Pholiota, strain, vegetative mycelium, antioxidant activity

Abstract

Aim. To determine the value of the antioxidant activity of the biomass and culture fluid extracts of strains of seven species of Pholiota genus: P. adiposa, P. alnicola, P. aurivella, P. limonella, P. nameko, P. squarrosa, P. subochracea, which stored in the Mushroom Culture Collection (IBK) of the M. G. Kholodny Institute of Botany of the National Academy of Sciences of Ukraine.
Materials and methods. The antioxidant properties of the biomass and culture fluid extracts of strains of Pholiota genus were determined by the method of Elfahri et al. using DPPH (1,1-diphenyl-2-picrylhydrazyl). Mycelia of the strains studied were grown by the surface method on a liquid glucose-pepton-yeast medium. The culture fluid was separated from the mycelial biomass by filtration through a capron filter. The absorption of methanol extracts of the culture fluid and the biomass of the strains studied was measured at 517 nm on a SF 46 LOMO spectrophotometer.
Results and discussion. Comparing the data obtained we can conclude that the antioxidant effect is significantly higher in the case of methanol biomass extracts – the indicators ranged from 65.98 ± 0.98 % (P. nameko) to 83.6 ± 1.4 % (P. alnicola). As for the culture fluid extracts, the maximum values were recorded in the case of P. limonella (38.3 ± 1.14 %), and the minimum values were observed for P. subochracea (7.37 ± 0.46 %).
Conclusions. For the first time, the value and limits of variation in the antioxidant activity of the biomass (65-83 %) and culture fluid extracts (7.4-38 %) have been determined for strains of medicinal fungal species P. adiposa, P. alnicola, P. aurivella, P. limonella, P. nameko, P. squarrosa, P. subochracea.

References

Pegler, D. N. Useful fungi of the world: the Shii-take, Shimeji, Enoki-take, and Nameko mushrooms. Mycologist 2003, 17 (1), 3-5. https://doi.org/10.1017/S0269915X03001071.

Gizaw, B. Cultivation and yield performance of Pholiota nameko on different agro industrial wastes. Academia Journal of Food Research 2015, 3 (3), 32-42.

Dulger, B. Antimicrobial activity of the macrofungus Pholiota adiposa. Fitoterapia 2004, 75 (3), 395-397. https://doi.org/10.1016/j.fitote.2004.01.005.

Nowacka, N.; Nowak, R.; Drozd, M.; Olech, M.; Los, R.; Malm, A. Analysis of phenolic constituents, antiradical and antimicrobial activity of edible mushrooms growing wild in Poland. LWT - Food Science and Technology 2014, 59 (2, Part 1), 689-694. https://doi.org/10.1016/j.lwt.2014.05.041.

Wang, C. R.; Zhou, R.; Ng, T. B.; Wong, J. H.; Qiao, W. T.; Liu, F. First report on isolation of methyl gallate with antioxidant, anti-HIV-1 and HIV-1 enzyme inhibitory activities from a mushroom (Pholiota adiposa). Toxicol. Pharmacol. 2014, 37 (2), 626-637. https://doi.org/10.1016/j.etap.2014.01.023.

Chou, C.-H.; Sung, T.-J.; Hu, Y.-N.; Lu, H.-Y.; Yang, L.-C.; Cheng, K.-C.; Lai, P.-S.; Hsieh, C.-W. Chemical analysis, moisture-preserving, and antioxidant activities of polysaccharides from Pholiota nameko by fractional precipitation. J. Biol. Macromol. 2019, 131, 1021-1031. https://doi.org/10.1016/j.ijbiomac.2019.03.154.

Zhu, D.; Guo, R.; Li, W.; Song, J.; Cheng, F. Improved Postharvest Preservation Effects of Pholiota nameko Mushroom by Sodium Alginate–Based Edible Composite Coating. Food and Bioprocess Technology 2019, 12 (4), 587-598. https://doi.org/10.1007/s11947-019-2235-5.

He, Y.; Wang, R.; Huang, B.; Dai, Q.; Lin, J. Pholiotone A, a new polyketide derivative from Pholiota sp. Natural Product Research 2020, 34 (14), 1957-1961. https://doi.org/10.1080/14786419.2019.1569005.

Sung, T.-J.; Wang, Y.-Y.; Liu, K.-L.; Chou, C.-H.; Lai, P.-S.; Hsieh, C.-W. Pholiota nameko Polysaccharides Promotes Cell Proliferation and Migration and Reduces ROS Content in H2O2-Induced L929 Cells. Antioxidants 2020, 9 (1), 65. https://doi.org/10.3390/antiox9010065.

Deng, P.; Zhang, G.; Zhou, B.; Lin, R.; Jia, L.; Fan, K.; Liu, X.; Wang, G.; Wang, L.; Zhang, J. Extraction and in vitro antioxidant activity of intracellular polysaccharide by Pholiota adiposa SX-02. Journal of Bioscience and Bioengineering 2011, 111 (1), 50-54. https://doi.org/10.1016/j.jbiosc.2010.08.004.

Sun, Z.; Tian, Y.; Jia, M.; Pang, L.; Deng, P.; Fan, K.; Liu, X.; Jia, S.; Jia, L. Extraction and in vitro antioxidant activity of exopolysaccharide by Pholiota adiposa SX-01. African Journal of Microbiology Research 2012, 6 (8), 1869-1876. https://doi.org/10.5897/AJMR12.012.

Liu, Y.; Sun, Y.; Huang, G. Preparation and antioxidant activities of important traditional plant polysaccharides. J. Biol. Macromol. 2018, 111, 780-786. https://doi.org/10.1016/j.ijbiomac.2018.01.086.

Zhu, Z.-Y.; Pan, L.-C.; Han, D.; Sun, H.-q.; Chen, L.-J. Structural properties and antioxidant activities of polysaccharide from fruit bodies of Pholiota nameko. Natural Product Research 2019, 33 (11), 1563-1569. https://doi.org/10.1080/14786419.2017.1423308.

Yu, S.; Jiang, J.; Li, W. Co-cultured Lepista sordida and Pholiota nameko polysaccharide-iron(III) chelates exhibit good antioxidant activity. RSC Adv. 2020, 10 (46), 27259-27265. https://doi.org/10.1039/D0RA03258A.

Zheng, L.; Ma, Y.; Zhang, Y.; Meng, Q.; Yang, J.; Wang, B.; Liu, Q.; Cai, L.; Gong, W.; Yang, Y.; Shi, J. Increased antioxidant activity and improved structural characterization of sulfuric acid-treated stepwise degraded polysaccharides from Pholiota nameko PN-01. J. Biol. Macromol. 2021, 166, 1220-1229. https://doi.org/10.1016/j.ijbiomac.2020.11.004.

Gan, D.; Ma, L.; Jiang, C.; Wang, M.; Zeng, X. Medium optimization and potential hepatoprotective effect of mycelial polysaccharides from Pholiota dinghuensis Bi against carbon tetrachloride-induced acute liver injury in mice. Food Chem. Toxicol. 2012, 50 (8), 2681-2688. https://doi.org/10.1016/j.fct.2012.05.003.

Zheng, L.; Zhai, G.; Zhang, J.; Wang, L.; Ma, Z.; Jia, M.; Jia, L. Antihyperlipidemic and hepatoprotective activities of mycelia zinc polysaccharide from Pholiota nameko SW-02. J. Biol. Macromol. 2014, 70, 523-529. https://doi.org/10.1016/j.ijbiomac.2014.07.037.

Kawagishi, H.; Abe, Y.; Nagata, T.; Kimura, A.; Chiba, S. A Lectin from the Mushroom Pholiota aurivella. Agricultural and Biological Chemistry 1991, 55 (10), 2485-2489. https://doi.org/10.1080/00021369.1991.10871000.

Clericuzio, M.; Piovano, M.; Chamy, M. C.; Garbarino, J. A.; Milanesio, M.; Viterbo, D.; Vidari, G.; Finzi, P. V. Structural characterisation of metabolites from Pholiota spumosa (Basidiomycetes). Croatica Chemica Acta 2004, 77 (4), 605-611. https://hrcak.srce.hr/102987.

Minato, K.; Kasahara, S. Immunomodulating action of edible mushrooms, Pleurotus cornucopiae var. citrinopileatus and Pholiota nameko. Congress Handbook & Abstracts Book 1, 8th International Mycological Congress, Cairns, Australia, 21–25 August, 2006; p. 219.

Minato, K.-I., Mushrooms: Immunomodulating Activity and Role in Health Promotion. In Dietary Components and Immune Function, Watson, R. R.; Zibadi, S.; Preedy, V. R., Eds. Humana Press: Totowa, NJ, 2010; pp 529-539. https://doi.org/10.1007/978-1-60761-061-8_28.

Medicinal Mushrooms: Recent Progress in Research and Development; Agrawal, D. C.; Dhansekaran, M., Eds.; Springer Nature Singapore Pte Ltd: Singapore, 2019. https://doi.org/10.1007/978-981-13-6382-5.

Nguyen, T. K.; Shin, D. B.; Lee, S. M.; Im, K. H.; Lee, T. S.; Lee, U. Y. Antioxidant and Anti-Inflammatory Activities of Methanol and Hot Water Extracts of Pholiota nameko Fruiting Bodies. The Korean Journal of Mycology 2013, 41 (2), 97-103. http://dx.doi.org/10.4489/KJM.2013.41.2.97.

Jo, S.-H.; Jin, G.; Yang, Y.; Jung, K.-J.; Yun, H.-S.; Yu, Y.; Park, -M. Physiological activity of Pholiota nameko sp. ethanol extract. Journal of Mushroom 2010, 8 (4), 142-149.

Ji, H.; Zhang, L.; Zhang, H.; Li, G.; Yang, M. Antioxidant activities of extracts from Pholiota nameko. Advanced Materials Research 2012, 343-344, 457-462. https://doi.org/10.4028/www.scientific.net/AMR.343-344.457.

Elmastas, M.; Isildak, O.; Turkekul, ; Temur, N. Determination of antioxidant activity and antioxidant compounds in wild edible mushrooms. Journal of Food Composition and Analysis 2007, 20 (3), 337-345. https://doi.org/10.1016/j.jfca.2006.07.003.

Pham-Huy, L. A.; He, H.; Pham-Huy, C. Free radicals, antioxidants in disease and health. Int J Biomed Sci 2008, 4 (2), 89-96.

Qian, L.; Zhang, Y.; Liu, F. Purification and characterization of a ∼43 kDa antioxidant protein with antitumor activity from Pholiota nameko. Sci. Food Agric. 2016, 96 (3), 1044-1052. https://doi.org/10.1002/jsfa.7194.

Bisko, N.; Lomberg, M.; Mykchaylova, O.; Mytropolska, N. IBK Mushroom Culture Collection. Version 1.4. The IBK Mushroom Culture Collection of the M. G. Kholodny Institute of Botany. Occurrence dataset https://doi.org/10.15468/dzdsqu accessed via GBIF.org on 2021-12-12.

Dyakov, M. Y.; Kamzolkina, O. V.; Shtaer, O. V.; Bis’ko, N. A.; Poedinok, N. L.; Mikhailova, O. B.; Tikhonova, O. V.; Tolstikhina, T. E.; Vasil’eva, B. F.; Efremenkova, O. V. Morphological characteristics of natural strains of certain species of basidiomycetes and biological analysis of antimicrobial activity under submerged cultural conditions. Microbiology 2011, 80 (2), 274. https://doi.org/10.1134/S0026261711020044.

Ebrahimzadeh, M. A.; Khalili, M.; Dehpour, A. A. Ethyl acetate and methanolic extracts from three algae and their potential antioxidant activity in vitro [Ekstrakty etilacetata i metanola iz trekh morskih vodoroslei i ih potentsial'naya antioksidantnaya aktivnost' in vitro, in Russian], Algologia 2019, 29 (1), 30-39. https://doi.org/10.15407/alg29.01.030.

Ayyash, M.; Johnson, S. K.; Liu, S.-Q.; Mesmari, N.; Dahmani, S.; Al Dhaheri, A. S.; Kizhakkayil, J. In vitro investigation of bioactivities of solid-state fermented lupin, quinoa and wheat using Lactobacillus spp. Food Chem. 2019, 275, 50-58. https://doi.org/10.1016/j.foodchem.2018.09.031.

Elfahri, K. R.; Vasiljevic, T.; Yeager, T.; Donkor, O. N. Anti-colon cancer and antioxidant activities of bovine skim milk fermented by selected Lactobacillus helveticus J. Dairy Sci. 2016, 99 (1), 31-40. https://doi.org/10.3168/jds.2015-10160.

Kim, J.-H. Physiological activities of water extract and solvent fractions of an edible mushroom, Pholiota adiposa. The Korean Journal of Mycology 2014, 42 (3), 207-212. https://doi.org/10.4489/KJM.2014.42.3.207.

Gong, C. Y.; Hu, Q. X.; Ji, Y. M. Antioxidation Study of Pholiota adiposa (Fr.) Quel and its Polysaccharides. Advanced Materials Research 2012, 345, 195-200. https://doi.org/10.4028/www.scientific.net/AMR.345.195.

Published

2021-12-23

How to Cite

(1)
Regeda, L. V.; Bisko, N. A.; Gurinovych, N. V. The Antioxidant Activity of Extracts of the Mycelium and the Culture Fluid of Medicinal Macromycetes of Pholiota (Fr.) P. Kumm. Genus. J. Org. Pharm. Chem. 2021, 19, 47-53.

Issue

Section

Original researches