DOI: https://doi.org/10.24959/ophcj.20.195619

Application of thin layer chromatography for the analytical diagnostics of combined prochlorperazine poisonings

Serhii I. Merzlikin, Tetiana V. Kucher, Volodymyr I. Stepanenko, Olena H. Pogosyan, Mykola Yu. Golik

Abstract


The uncontrolled simultaneous application of prochlorperazine with typical phenothiazine derivatives antipsychotics and other drugs leads to fatal poisonings.

Aim. To optimize TLC procedures for the screening examinations in combined poisonings with prochlorperazine and other drugs.

Results and discussion. The chromatographic mobility of prochlorperazine, clorpromazine, ibuprofen, trifluoperazine and quetiapine has been studied on two types of plates (HPTLC and TLC). It has been shown that the TLC-systems used (special – for prochlorperazine, general – for screening of phenothiazine derivatives, as well as drug and narcotic substances of the basic nature recommended by the International Association of Forensic Toxicologists) rather effectively separate the substances analyzed in a thin layer of a sorbent, except for trifluoperazine. To detect the adsorption zones of substances, the Lieberman reagent giving different staining, the Dragendorff reagent and irradiation of chromatograms by UV-light (λ = 254 nm) have been proposed. The detection sensitivity is 0.5 – 1.0 μg in the sample. The validation assessment of the methods developed has been carried out by the chromatographic analysis of prochlorperazine model mixtures with each of the substances studied.

Experimental part. The studies were carried out on HPTLC plates (silica gel 60G, particle size – 5 – 7 μm, substrate type – glass, Estonia) with 10 × 10 cm in size and Merck plates (silica gel 60G F254, particle size – 10 – 15 μm, substrate type – aluminum foil, Germany) with 10 × 10 cm in size.

Conclusions. The TLC-conditions for screening of prochlorperazine, chlorpromazine, ibuprofen, trifluoperazin and quetiapine have been determined; they can be recommended for drugs detection in real samples of biological objects in the case of combined poisoning.

Received: 17.02.2020
Revised: 15.04.2020
Accepted: 29.05.2020


Keywords


prochlorperazine; poisoning; thin layer chromatography; chromogenic reagents; solvent systems

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Abbreviated key title: J. Org. Pharm. Chem.

ISSN 2518-1548 (Online), ISSN 2308-8303 (Print)