The enzymatic method for the quantitative determination of benzalkonium chloride in the antiseptic solution “CUTASEPT® F”

Authors

DOI:

https://doi.org/10.24959/ophcj.21.244359

Keywords:

benzalkonium chloride, CUTASEPT® F, cholinesterase, acetylcholine

Abstract

Aim. To develop an alternative method for the quantitative determination of the benzalkonium chloride content as an active pharmaceutical ingredient in the disinfectant solution “CUTASEPT® F”.
Materials and methods. The method is based on the ability of benzalkonium chloride to inhibit the enzymatic hydrolysis of acetylcholine by acetylcholinesterase. The reaction rate is assessed by the non-hydrolyzed acetylcholine residue, which is determined by the amount of peracetic acid produced during the interaction with the excess of the hydrogen peroxide solution. The indicator reaction is the interaction of p-phenetidine with peracetic acid that leads to the formation of 4,4’-azoxyphenetole with λmax = 358 nm (log10 ε = 4.2).
Results and discussion. As a result of the research conducted the linear dependence of the degree of inhibition of the enzymatic hydrolysis of acetylcholine (U, %) on the concentration of benzalkonium chloride was determined in the concentration range of (0.5 – 7.0) × 10–6 mol L-1 with the correlation coefficient of 0.999. The limit of quantitation was 1.9 × 10–6 mol L-1.
Conclusions. As a result of the research conducted the kinetic enzymatic method for the quantitative determination of benzalkonium chloride has been developed by its inhibitory effect in the biochemical reaction of acetylcholine hydrolysis. This method is fast, cheap and easy to perform, does not require expensive equipment, and available for use in the field.

Supporting Agency

  • The work is a part of researches of the National University of Pharmacy on the topic «Organic synthesis and analysis of biologically active compounds, drugs development on the basis of synthetic substances» (the state registration No. 01144000943; the research period 2019 – 2024).

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References

  1. Ghafoor, D.; Khan, Z.; Khan, A.; Ualiyeva, D.; Zaman, N. Excessive use of disinfectants against COVID-19 posing a potential threat to living beings. Current Research in Toxicology 2021, 2, 159-168. https://doi.org/10.1016/j.crtox.2021.02.008.
  2. МЕТОДИЧНІ ВКАЗІВКИ щодо застосування засобу КУТАСЕПТ® Г (CUTASEPT® G) з метою дезінфекції. http://www.xn--80aaolbmrsqie.com.ua/upl/admin_upload/bode/%D1%81utasept_g.pdf (accessed Sep 5, 2021).
  3. Martykanova, D. S.; Davletova, N. C.; Zemlenuhin, I. A.; Volchkova, V. I.; Mugallimov, S. M.; Ahatov, A. M.; Laikov, A. V.; Markelova, M. I.; Boulygina, E. A.; Lopukhov, L. V.; Grigoryeva, T. V. Skin Microbiota in Contact Sports Athletes and Selection of Antiseptics for Professional Hygiene. BioMed Research International 2019, Article ID 9843781. https://doi.org/10.1155/2019/9843781.
  4. Gaber, M.; Abu Shawish, H. M.; Khedr, A. M.; Abed-Almonem, K. I. Determination of benzalkonium chloride preservative in pharmaceutical formulation of eye and ear drops using new potentiometric sensors. Materials Science and Engineering: C 2012, 32 (8), 2299-2305. https://doi.org/10.1016/j.msec.2012.06.018.
  5. Bertuzzi, T.; Pietri, A. Determination of Benzalkonium Homologues and Didecyldimethylammonium in Powdered and Liquid Milk for Infants by Hydrophilic Interaction Liquid Chromatography–Mass Spectrometry. Food Analytical Methods 2014, 7 (6), 1278-1284. https://doi.org/10.1007/s12161-013-9745-y.
  6. Díez, C.; Feinberg, M.; Spörri, A. S.; Cognard, E.; Ortelli, D.; Edder, P.; Rudaz, S. Evaluation of Quantification Methods to Compensate for Matrix Effects in the Analysis of Benzalkonium Chloride and Didecyldimethylammonium Chloride in Fruits and Vegetables by LC-ESI-MS/MS. Food Analytical Methods 2016, 9 (2), 485-499. https://doi.org/10.1007/s12161-015-0216-5.
  7. Miyauchi, T.; Mori, M. Improved quantitative determination of benzalkonium chloride in treated wood by liquid chromatography. Holzforschung 2007, 61 (3), 337-341. https://doi.org/10.1515/HF.2007.047.
  8. AlAani, H.; AlNukkary, Y. Determination of Benzalkonium Chloride in Ophthalmic Solutions by Stability-Indicating HPLC Method: Application to a Stability Study. Journal of Applied Pharmaceutical Science 2016, 6 (5), 080-089. https://doi.org/10.7324/JAPS.2016.60513.
  9. Issa, Y. M.; Abdel-kader, N. S.; Zahran A. E. Spectrophotometric Determination of Benzalkonium Chloride using Sulfonephthaleins. Int. J. Pharm. Sci. Rev. Res. 2021, 68 (1), 50-59. http://dx.doi.org/10.47583/ijpsrr.2021.v68i01.009.
  10. Kostić, D. A.; Mitić, S. S.; Nasković, D. Č.; Zarubica, A. R.; Mitic, M. N. Determination of Benzalkonium Chloride in Nasal Drops by High-Performance Liquid Chromatography. E-Journal of Chemistry 2012, 9, Article ID 172070. https://doi.org/10.1155/2012/172070.
  11. Ivan, G. R.; Stoica, R.; Radu, E.; Capra, L.; Manolache, M. Determination of Benzalkonium Chloride from Biocide Products: Selectivity Study. Proceedings 2019, 29 (1), 94. https://doi.org/10.3390/proceedings2019029094.
  12. Derzhavna farmakopeia Ukrainy: v 3 tomakh, 2 vydannia [The State Pharmacopoeia of Ukraine: in 3 volumes, 2nd ed., in Ukrainian]; State Enterprise “Ukrainian Scientific Pharmacopoeial Center for Quality of Medicines”: Kharkiv, 2015; Vol. 1.
  13. Blazheevskiy, M. Ye.; Dyadchenko, V. V. Kinetic determination of anticholinesterase compounds by a biochemical method by oxidizing reaction of p-phenetidine as display. Farmatsevtychnyi zhurnal 2004, 2, 52-58.

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Published

2022-03-23

How to Cite

(1)
Koval’ska, O. V.; Blazheyevskіy M. Y. The Enzymatic Method for the Quantitative Determination of Benzalkonium Chloride in the Antiseptic Solution “CUTASEPT® F.” J. Org. Pharm. Chem. 2022, 19, 33-39.

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Original Researches