Determination of the extraction frequency of green tea leaves by the antioxidant method

Authors

DOI:

https://doi.org/10.24959/ophcj.22.252320

Keywords:

green tea, leaves, analysis, extract, extraction, antioxidant activity

Abstract

Aim. To determine the optimal extraction frequency of green tea leaves with 60 % ethanol by the antioxidant method.
Materials and methods. Chun Myn green tea leaves were the object of the study, the raw material was collected in Anhui province (China) from March to April. Dry green tea leaves were standardized according to the European Pharmacopeia 9.0. Spectrophotometry was used to quantify biologically active substances. The antioxidant activity was determined by the potentiometric method. Potentiometric measurements were performed on a HANNA 2550 pH meter (Germany) with a combined platinum EZDO 50 PO electrode (Taiwan). A UV-1000 spectrophotometer (China) was used to measure the optical density.
Results and discussion. The total content of phenolic compounds was 9.60 ± 0.17, 1.30 ± 0.03 and 0.12 ± 0.002 %, catechins – 9.20 ± 0.18, 1.20 ± 0.02 and 0.07 ± 0.002 %, flavonoids – 0.27 ± 0.005, 0.04 ± 0.001, 0.005 ± 0.001, hydroxycinnamic acids – 0.49 ± 0.01, 0.07 ± 0.002 and 0.007 ± 0.001 %, dry residue – 10.75 ± 0.11, 1.59 ± 0.02 and 0.15 ± 0.002 %, the antioxidant activity was 474.08 ± 9.48, 67.70 ± 1.35 and 7.01 ± 0.14 mmol-equiv mdry res-1 for the first, second and third extraction, respectively. According to the results obtained, the optimal number of extractions of the raw material with 60 % ethanol was found to be two.
Conclusions. The dynamic of extractions of biologically active substances of green tea leaves has been studied by triple extraction of the raw material to find the optimal extraction frequency; for the first time, a method for determining the extraction frequency based on the antioxidant activity of the extracts has been developed and proposed. It has been found that the optimal extraction rate is 2 times. The results obtained will be used in the further production of herbal medicines, dietary supplements, and cosmetic products with a green tea extract.

Supporting Agency

  • The work is a part of the research of the National University of Pharmacy on the topic “Organic synthesis and analysis of bio- logically active compounds, drugs development based on synthetic substances” (the state registration No. 01144000943; the research period 2019 – 2024).

Downloads

Download data is not yet available.

References

  1. Forman, H. J.; Zhang, H. Targeting oxidative stress in disease: promise and limitations of antioxidant therapy. Nature Reviews Drug Discovery 2021, 20 (9), 689-709. 10.1038/s41573-021-00233-1.
  2. García-Sánchez, A.; Miranda-Díaz, A. G.; Cardona-Muñoz, E. G. The Role of Oxidative Stress in Physiopathology and Pharmacological Treatment with Pro- and Antioxidant Properties in Chronic Diseases. Oxidative Medicine and Cellular Longevity 2020, 2020, 2082145. 10.1155/2020/2082145.
  3. Maslov, O. Y.; Kolisnyk, S. V.; Komisarenko, M. A.; Kolisnyk, O. V.; Ponomarenko, S. V. Antioxidant activity of green tea leaves (Camellia Sinensis) liquid extracts. Pharmacologyonline 2021, 3, 291-298.
  4. Maslov, O. Yu.; Kolisnyk, S. V.; Komisarenko, M. A.; Altukhov, A. A.; Dynnyk, K. V.; Stepanenko, V. I. Study and evaluation of the antioxidant activity of dietary supplements with green tea extract. Current issues in pharmacy and medicine: science and practice 2021, 14 (2), 215-219. https://doi.org/10.14739/2409-2932.2021.2.233306.
  5. Maslov, O. Yu.; Komisarenko, M. A.; Kolisnyk, Yu. S.; Kostina, T. A. Determination of catechins in green tea leaves by HPLC compared to spectrophotometry. Journal of Organic and Pharmaceutical Chemistry 2021, 19 (3), 28-33. https://doi.org/10.24959/ophcj.21.238177.
  6. Maslov, O. Yu.; Kolisnyk, S. V.; Komisarenko, M. A.; Akhmedov, E. Yu.; Poluian, S. M.; Shovkova, Z. V. Study of flavonoids and phenolic acids in green tea leaves. Current issues in pharmacy and medicine: science and practice 2021, 14 (3), 287-291. https://doi.org/10.14739/2409-2932.2021.3.240287.
  7. European Pharmacopoeia, 9th; European Directorate for the Quality of Medicines & HealthCare: Strasbourg, 2016.
  8. Derzhavna farmakopeia Ukrainy: v 3 tomakh, 2 vydannia [The State Pharmacopoeia of Ukraine: in 3 volumes, 2nd, in Ukrainian]; State Enterprise “Ukrainian Scientific Pharmacopoeial Center for Quality of Medicines”: Kharkiv, 2014; Vol. 2.
  9. Al-Shwaiyat, M.; Denisenko, T.; Miekh, Yu.; Vishnikin, A. Spectrophotometric determination of polyphenols in green teas with 18-molybdodiphosphate. Chemistry & Chemical Technology 2018, 12 (2), 135-142. https://doi.org/10.23939/chcht12.02.135.
  10. Fernandes, A. J. D.; Ferreira, M. R. A.; Randau, K. P.; de Souza, T. P.; Soares, L. A. L. Total Flavonoids Content in the Raw Material and Aqueous Extractives from Bauhinia monandra Kurz (Caesalpiniaceae). The Scientific World Journal 2012, 2012, 923462. https://doi.org/10.1100/2012/923462.
  11. Štefan, M. B.; Vuković Rodríguez, J.; Blažeković, B.; Kindl, M.; Vladimir-Knežević, S. Total Hydroxycinnamic Acids Assay: Prevalidation and Application on Lamiaceae Species. Food Analytical Methods 2014, 7 (2), 326-336. 10.1007/s12161-013-9630-8.
  12. Maslov, O. Yu.; Kolisnyk, S. V.; Ponomarenko, S. V.; Ahmedov, E. Yu.; Shovkova, Z. V. The study of the effect of ethyl alcohol concentrations on the antioxidant activity of ascorbic acid solutions. Journal of Organic and Pharmaceutical Chemistry 2021, 19 (2), 44-47. https://doi.org/10.24959/ophcj.21.231947.
  13. Maslov, O. Yu.; Kolisnyk, S. V.; Komissarenko, N. A.; Kostina, T. A. Development and validation of the potentiometric method for determination of antioxidant activity of epigallocatechin-3-O-gallate. Pharmacologyonline 2021, 2, 35-42.
  14. Chou, S.; Lai, C.; Lin, C.; Shih, Y. Study of the chemical composition, antioxidant activity and anti-inflammatory activity of essential oil from Vetiveriazizanioides. Food Chemistry2012134(1), 262-268. https://doi.org/10.1016/j.foodchem.2012.02.131.
  15. Maslov, O.; Kolisnyk, S.; Komisarenko, M.; Golik, M. Study of total antioxidant activity of green tea leaves (Camellia sinensis L.). Herba Polonica, 2022, 68 (1), 1-9. https://doi.org/10.2478/hepo-2022-0003.

Downloads

Published

2022-05-30

How to Cite

(1)
Maslov, O. Y.; Komisarenko , M. A.; Kolisnyk, S. V.; Golik, M. Y.; Tsapko, Y. O.; Akhmedov, E. Y. Determination of the Extraction Frequency of Green Tea Leaves by the Antioxidant Method. J. Org. Pharm. Chem. 2022, 20, 28-34.

Issue

Section

Original Researches