Application of the enzymatic method for the quantitative determination of dequalinium chloride in lozenges

Authors

DOI:

https://doi.org/10.24959/ophcj.22.252499

Keywords:

dequalinium chloride, cholinesterase, acetylcholine, quantification

Abstract

Aim. To develop a new kinetic spectrophotometric enzymatic method suitable for the quantitative determination of dequalinium chloride in lozenges.
Materials and methods. An enzymatic kinetic spectrophotometric method for the quantitative determination of dequalinium chloride in lozenges  has been proposed. It is based on the ability of dequalinium chloride to inhibit the enzymatic hydrolysis reaction of acetylcholine by cholinesterase in the presence of the acetylcholine (AСh) excess and H2O2. The degree of inhibition was determined by the kinetic method using two conjugated reactions: ACh perhydrolysis (interaction with an excess of hydrogen peroxide) followed by oxidation with the peroxyacid formed. Peracetic acid formed in situ by the reaction between unreacted ACh and H2O2 interacts with p-phenetidine forming a product, which absorbs at λmax = 354 nm, in the phosphate buffer solution with pH 8.3 at room temperature.
Results and discussion. The linear dependence of the calibration graph for the quantitative determination of dequalinium chloride was in the concentration range of 0.2 – 0.8 μg mL–1 (r = 0.999). LOD and LOQ were 0.01 × 10–6 and 0.03 × 10–6 mol L–1, respectively. For the quantitative determination of dequalinium chloride in lozenges, RSD ≤ 2.65 % (accuracy, δ = -1.10…+1.78 %).
Conclusions. A new enzymatic kinetic spectrophotometric method has been developed, and its applicability to the quantitative determination of dequalinium chloride in lozenges has been shown. It does not require a complicated treatment of the analyte and a tedious extraction procedure. The method proposed is sensitive enough to determine a small amount of the active pharmaceutical ingredient. These advantages encourage the application of the method proposed in routine quality control of the drugs studied in analytical laboratories.

Supporting Agency

  • The work is a part of the research of the National University of Pharmacy on the topic “Organic synthesis and analysis of biologically active compounds, drug development based on synthetic substances” (the state registration No. 01144000943; the research period 2019 – 2024).

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References

  1. Bailly, C. Medicinal applications and molecular targets of dequalinium chloride. Biochem Pharmacol 2021, 186, 114467. https://doi.org/10.1016/j.bcp.2021.114467.
  2. Abaturov, O. Y.; Ahafonova, O. O.; Tokarieva, N. M. Application of lysozyme and dequalinium chloride in the treatment of acute respiratory diseases of the upper respiratory tract in children. CHILD`S HEALTH 2021, 13 (6), 576-584. https://doi.org/10.22141/2224-0551.13.6.2018.143163.
  3. 3. Tekgunduz, S. E.; Saracoglu, F. Evaluation of the Effectiveness of Dequalinium Chloride Vaginal Tablets in Aerobic Vaginitis: A Placebo-Controlled Study. Eastern Journal of Medical Sciences 2021, 6 (3), 50-56. https://doi.org/10.32677/ejms.v6i3.3090.
  4. Pan, Y.; Zhao, S.; Chen, F. The potential value of dequalinium chloride in the treatment of cancer: Focus on malignant glioma. Clinical and Experimental Pharmacology and Physiology 2021, 48 (4), 445-454. https://doi.org/10.1111/1440-1681.13466.
  5. Macé, S.; Truelstrup Hansen, L.; Rupasinghe, H. P. V. Anti-Bacterial Activity of Phenolic Compounds against Streptococcus pyogenes. Medicines 2017, 4 (2), 25. https://doi.org/10.3390/medicines4020025.
  6. European Pharmacopoeia, 6th Ed.; European Directorate for the Quality of Medicines: Strasbourg, 2008.
  7. British Pharmacopoeia; The Stationery Office: London, 2008.
  8. Taylor, R. B.; Toasaksiri, S.; G. Reid, R.; Wood, D. Determination of the Quaternary Ammonium Compounds Dequalinium and Cetylpyridinium Chlorides in Candy-based Lozenges by High-performance Liquid Chromatography. Analyst 1997, 122 (9), 973-976. https://doi.org/10.1039/A703893C.
  9. 9. Taylor, R. B.; Toasaksiri, S.; Reid, R. G. Capillary electrophoresis and liquid chromatography in the analysis of some quaternary ammonium salts used in lozenges as antibacterial agents. Journal of capillary electrophoresis 1998, 5 (1-2), 45-50.
  10. Chen, F.-A.; Wu, K.-S.; Huang, M.-C.; Chen, C.-Y.; Wu, A.-B. Simultaneous quantitation of cationic disinfectants by high-performance liquid chromatography on a silica gel column using aqueous eluents. Journal of Food and Drug Analysis 2001, 9 (4), Article 4. https://doi.org/10.38212/2224-6614.2780.
  11. 11. Fang, W.-H; Liu, J., Shen, J.; Zhang, L.-J. Determination of dequalinium chloride in dequalinium chloride buccal tablets by HPLC. Anhui Medical and Pharmaceutical Journal 2011, 7, 837–839.
  12. Mohamed, H. A. Spectrophotometric Determination of Dequalinium Chloride. Anal. Lett. 1993, 26 (11), 2421-2429. https://doi.org/10.1080/00032719308017481
  13. Leung, C. P.; Kwan, S. Y. Spectrophotometric determination of dequalinium chloride in pharmaceutical preparations. Analyst 1979, 104 (1235), 143-146. https://doi.org/10.1039/AN9790400143.
  14. Mohamed, T. Y.; Dossouki, H. A.; Moustafa, M. M.; Ghoname. M. S. Spectrophotometric methods for the determination of dequalinium chloride and clidinium bromide using ion-pair complex formation with acid dyes. Egyptian Journal of Chemistry, 2008, 51 (1), 113–123.
  15. Blazheyevskiy, M. Ye.; Koval’ska, O. V. Application of the kinetic enzymatic method for benzalkonium chloride determination in an aerosol preparation. GISAP: Medical Sciences, Pharmacology 2017, 13, 33-35.
  16. 16. Derzhavna farmakopeia Ukrainy: v 3 tomakh, 2 vydannia [The State Pharmacopoeia of Ukraine: in 3 volumes, 2nd , in Ukrainian]; State Enterprise “Ukrainian Scientific Pharmacopoeial Center for Quality of Medicines”: Kharkiv, 2015; Vol. 1.
  17. Diadchenko, V. V.; Petrukhin, S. Yu.; Novikov, O. I. Boiovi toksychni khimichni rechovyny: v 3 tomakh [Fighting toxic chemicals: in 3 volumes, in Ukrainian]; Brovin O. V.: Kharkiv, 2018; Vol. 1.
  18. Blazheevskiy, M. E.; Kovalska, O. V.; Dyadchenko, V. V. (National University of Pharmacy, Kharkiv, Ukraine). Sposib vyznachennia aktyvnosti kholinesterazy krovi [Method for determining blood cholinesterase activity, in Ukrainian]. Ukraine Patent 117474, 26.06.2017.
  19. Koval’ska, O. V.; Blazheyevskіy M. Y. The Enzymatic Method for the Quantitative Determination of Benzalkonium Chloride in the Antiseptic Solution “CUTASEPT® F.” Journal of Organic and Pharmaceutical Chemistry 2021, 19 (4), 33-39. https://doi.org/10.24959/ophcj.21.244359.

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Published

2022-05-30

How to Cite

(1)
Blazheyevskіy M. Y.; Koval’ska, O. V. Application of the Enzymatic Method for the Quantitative Determination of Dequalinium Chloride in Lozenges. J. Org. Pharm. Chem. 2022, 20, 21-27.

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Original Researches