The quantitative determination of glutathione by the effect of the chemiluminescence inhibition in the catalytic oxidation reaction of luminol with hydrogen peroxide in the presence of hemoglobin

Authors

DOI:

https://doi.org/10.24959/ophcj.22.255967

Keywords:

glutathione, chemiluminescence inhibition, quantification, luminol

Abstract

Aim. To develop a method for the quantitative determination of reduced glutathione in a lyophilized powder for the preparation of “Hepaval®” solution for intravenous and intramuscular administration by the effect of inhibiting chemiluminescence in the luminol (H2L)–Н2О2–hemoglobin (Hb) system.
Materials and methods. The study object was the reduced glutathione substance and lyophilized powder for the preparation of “Hepaval®” solution for injection, ampoules of 4 mL No. 10 manufactured by “Valartin pharma” (Italy). The glutathione content in powder was determined using the chemiluminescence method by the effect of inhibiting the luminol oxidation reaction with hydrogen peroxide in the presence of Hb as a catalyst. The analysis was performed by the standard addition method.
Results and discussion. As a result of the studies, it has been found that under optimal conditions glutathione shows a noticeable inhibitory effect on chemiluminescence in the H2L–H2O2–Hb system. This phenomenon was used to develop a new procedure for the quantitative determination of glutathione in the substance and lyophilized powder for the preparation of “Hepaval®” aqueous solution for injection. The linear dependence of the integral chemiluminescence intencity (S) on the molar concentration of glutathione was maintained in the concentration range of (2 – 20) × 10–7 mol L-1. The graph equation was S = (–1.6 ± 0.2) × 107 × c + (198.9 ± 2.0), (r = 0.999). The relative standard deviation (RSD) was ±1.82 % (n = 7, P = 0.95).
Conclusions. The method has been developed, and the possibility of the quantitative determination of glutathione in powder for the preparation of “Hepaval®” solution for injection by the method of the chemiluminescence inhibition of the H2L–Н2О2–Нb system has been shown. The content of glutathione in powder calculated with reference to dried substance was 91.49 % (against 90.8 % by the certificate). The accuracy was +0.76 %.

Supporting Agency

  • The work is a part of the research of the National University of Pharmacy on the topic “Organic synthesis and analysis of biologically active compounds, drug development based on synthetic substances” (the state registration No. 01144000943; the research period 2019 – 2024).

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Published

2022-05-30

How to Cite

(1)
Blazheyevskіy M. Y.; Bondarenko, N. Y.; Mozgova, O. O. The Quantitative Determination of Glutathione by the Effect of the Chemiluminescence Inhibition in the Catalytic Oxidation Reaction of Luminol With Hydrogen Peroxide in the Presence of Hemoglobin. J. Org. Pharm. Chem. 2022, 20, 51-56.

Issue

Vol. 20 No. 1(77) (2022)

Section

Original Researches

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