The Quantitative Determination of Oxacillin Using Kinetiс-Spectrophotometric and Redox Titration Methods

Authors

DOI:

https://doi.org/10.24959/ophcj.23.273654

Keywords:

oxacillin, potassium hydrogen peroxomonosulfate, iodometry, kinetic spectrophotometry

Abstract

The search for new analytical reactions and finding out the optimal conditions for their course, which can be used as the basis for quantitative analytical determinations of penicillins, is a very urgent task.
Aim. To develop methods for the quantitative determination of Oxacillin.
Materials and methods. The study object was Oxacillin sodium salt powder in vials for preparing a solution for injections
(0.5 g). Peroxomonosulfate acid as triple potassium salt 2КНSO5∙КНSO4∙K2SO4 (Oxone®) of “extra pure” grade was used as an oxidant. The methods of kinetic spectriphotometry and redox titration were used.
Results and discussion. A simple procedure for the quantitative determination of the Oxacillin pure substance by the kinetic spectrophotometry and redox titration methods using potassium hydrogen peroxomonosulfate (KHSO5) has been developed. The results of the drug analysis obtained by newly developed and current methods are in good agreement with each other; δ (correctness) = (0.45 – 0.86) %.
Conclusions. Using the methods of kinetic spectrophotometric and redox titration, two independent procedures for the
quantitative determination of oxacillin in the substance and the drug product have been developed using potassium hydrogen peroxomonosulfate as an analytical reagent (KHSO5). A relative standard deviation RSD = (1.24 – 2.17) %.

Supporting Agency

  • The work is a part of the research of the National University of Pharmacy on the topic “Organic synthesis and analysis of biologically active compounds, drug development based on synthetic substances” (the state registration No. 01144000943; the research period of 2019 – 2024).

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References

  1. The United States Pharmacopeia USP38; United States Pharmacopeial Convention: Rockville, MD, 2015.
  2. The British Pharmacopeia; The Stationery Office: London, 2014.
  3. The United States Pharmacopeia USP30, the national formulary NF25; United States Pharmacopeial Convention: Rockville, MD, 2008.
  4. Wang, P.; Liu, H. L.; Wang, B.; Cheng, X. W.; Chen, Q. H.; Fu, H. A Method for Determination of Penicillin G Residue in Waste Penicillium chrysogenum Using High Performance Liquid Chromatography. Applied Mechanics and Materials 2015, 768, 15-24. https://doi.org/10.4028/www.scientific.net/AMM.768.15.
  5. Liu, K.; Sun, D.-w.; Zhao, Y. Assay detection for azlocillin sodium and sulbactam sodium for injection by HPLC. Chinese Journal of Pharmaceutical Analysis 2008, 28 (9), 1568-1570.
  6. Shafique Ahmad, A.; Rahman, N.; Islam, F. Spectrophotometric Determination of Ampicillin, Amoxycillin, and Carbenicillin Using Folin-Ciocalteu Phenol Reagent. J. Anal. Chem. 2004, 59 (2), 119-123. https://doi.org/10.1023/B:JANC.0000014736.59554.5c.
  7. Díaz-Bao, M.; Barreiro, R.; Miranda, J. M.; Cepeda, A.; Regal, P. Fast HPLC-MS/MS Method for Determining Penicillin Antibiotics in Infant Formulas Using Molecularly Imprinted Solid-Phase Extraction. Journal of Analytical Methods in Chemistry 2015, 2015, 959675. https://doi.org/10.1155/2015/959675.
  8. Puig, P.; Borrull, F.; Calull, M.; Aguilar, C. Sample stacking for the analysis of eight penicillin antibiotics by micellar electrokinetic capillary chromatography. Electrophoresis 2005, 26 (4-5), 954-961. https://doi.org/10.1002/elps.200406175.
  9. Batrawi, N.; Wahdan, S.; Al-Rimawi, F. A Validated Stability-Indicating HPLC Method for Simultaneous Determination of Amoxicillin and Enrofloxacin Combination in an Injectable Suspension. Scientia Pharmaceutica 2017, 85 (1), 6. https://doi.org/10.3390/scipharm85010006.
  10. Kipper, K.; Barker, C. I. S.; Standing, J. F.; Sharland, M.; Johnston, A. Development of a Novel Multipenicillin Assay and Assessment of the Impact of Analyte Degradation: Lessons for Scavenged Sampling in Antimicrobial Pharmacokinetic Study Design. Antimicrob. Agents Chemother. 2018, 62 (1), e01540-17. https://doi.org/10.1128/AAC.01540-17.
  11. Shapiro, A. B. Investigation of β-lactam antibacterial drugs, β-lactamases, and penicillin-binding proteins with fluorescence polarization and anisotropy: a review. Methods and Applications in Fluorescence 2016, 4 (2), 024002. https://doi.org/10.1088/2050-6120/4/2/024002.
  12. Navarro, M.; Li, M.; Müller-Bunz, H.; Bernhard, S.; Albrecht, M. Donor-Flexible Nitrogen Ligands for Efficient Iridium-Catalyzed Water Oxidation Catalysis. Chem. Eur. J. 2016, 22 (20), 6740-6745. https://doi.org/10.1002/chem.201600875.
  13. Khare, B.; Khare, K. Spectrophotometric Determination of Antibiotic Drug Penicillin in Pharmaceutical Samples Using 2,6-Dichlorophenol Indophenol, N-Bromocaprolactam and N-Chlorosuccinimide. International Journal of Recent Research in Physics and Chemical Sciences 2017, 4 (1), 1-7.
  14. Sangeetha, S.; Kumar, M.; Kumudhavalli, M.; Alexandar, S.; Jaykar, B. Development and Validation of UV Spectrophotometric Area Under Curve Method for Quantitative Estimation of Piperacillin and Tazobactam. International Journal of ChemTech Research 2017, 10 (2), 988-994.
  15. Sallach, J. B.; Snow, D.; Hodges, L.; Li, X.; Bartelt-Hunt, S. Development and comparison of four methods for the extraction of antibiotics from a vegetative matrix. Environ. Toxicol. Chem. 2016, 35 (4), 889-897. https://doi.org/10.1002/etc.3214.
  16. Keskar, M.R.; Jugade, R.M. Spectrophotometric Investigations of Macrolide Antibiotics: A Brief Review. Anal. Chem. Insights 2015, 10, ACI.S31857.https://doi.org/10.4137/ACI.S31857.
  17. Chen, J., Fang, C., Xia, W., Huang, T., & Huang, C.-H. Selective Transformation of β-Lactam Antibiotics by Peroxymonosulfate: Reaction Kinetics and Nonradical Mechanism. Environ. Science and Technology 2018, 52 (3), 1461–1470. https://doi.org/10.1021/acs.est.7b05543.

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Published

2023-08-30

How to Cite

(1)
Karpova, S. P.; Ivashura, M. M.; Koval, A. O.; Kolisnyk, Y. S. The Quantitative Determination of Oxacillin Using Kinetiс-Spectrophotometric and Redox Titration Methods. J. Org. Pharm. Chem. 2023, 21, 15-20.

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Original Researches