Metrological Characteristics of the Potentiometric Assay Developed for Determining the Antioxidant Activity of Ascorbic Acid




antioxidant activity, potentiometric method, validation, ascorbic acid


The potentiometric assay for determining the antioxidant activity of ascorbic acid has been developed and validated according to the following parameters: specificity, linearity, accuracy, repeatability, intermediate precision. The linearity was in the concentration range of 0.002 – 0.02 mol L–1 (r2 = 0.9993). The percentage of recovery was found to be in the range from 95.38 to 105.00 %. The values of %RSD for repeatability and intermediate precision were 1.86 and 1.95 %, respectively. The method is accurate and reliable, with the relative standard deviation of less than 2 %. It has been proven that the method developed is express, rapid, highly sensitive, accurate and sufficiently reliable.

Supporting Agency

  • The work is a part of the research of the National University of Pharmacy on the topic “Organic synthesis and analysis of biologically active compounds, drug development based on synthetic substances” (the state registration No. 01144000943; the research period of 2019 – 2024)


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How to Cite

Maslov, O. Y.; Kolisnyk, S. V.; Komisarenko, M. A.; Akhmedov, E. Y.; Koval, A. O.; Kostina, T. A.; Kolisnyk, O. V. Metrological Characteristics of the Potentiometric Assay Developed for Determining the Antioxidant Activity of Ascorbic Acid. J. Org. Pharm. Chem. 2023, 21, 31-37.



Original Researches