The Study of the Antibacterial Effect of the Umbellate Wintergreen Extract

Authors

  • Oleksiy V. Kovregin National University of Pharmacy of the Ministry of Health of Ukraine, Ukraine
  • Tetyana P. Osolodchenko Mechnikov Institute of Microbiology and Immunology of the National Academy of Medical Sciences of Ukraine, Ukraine https://orcid.org/0000-0001-7258-3880
  • Inna M. Vladymyrova National University of Pharmacy of the Ministry of Health of Ukraine, Ukraine https://orcid.org/0000-0002-4846-8839

DOI:

https://doi.org/10.24959/ophcj.24.324652

Keywords:

umbellate wintergreen, liquid extract, antimicrobial effect

Abstract

The article presents the studies on the antimicrobial effect of the umbellate wintergreen herb extract. The study object was the umbellate wintergreen herb extract obtained with 50% ethyl alcohol. The sensitivity of microbial strains to the umbellate wintergreen extract was determined by the well diffusion method in Mueller Hinton agar. Five test strains were used as test cultures: Staphylococcus aureus ATCC 6538 gram-positive microorganisms, Bacillus subtilis ATCC 6633 spore culture, gram-negative Proteus vulgaris ATCC 4636, Escherichia coli ATCC 25922, and Pseudomonas aeruginosa ATCC 9027. The antifungal effect was determined against Candida albicans ATCC 885-653. The antibacterial activity of the test substances was assessed by the diameter of the growth inhibition zones. It was found that the extract of the umbellate wintergreen herb exhibited antibacterial properties against test microorganisms where the diameters of the growth inhibition zones were at the level of 20-28 mm. The antibacterial effect of the umbellate wintergreen herb extract was also determined against such clinical strains of microorganisms as Staphylococcus aureus 124, Enterococcus faecalis 42, Pseudomonas aeruginosa 18, Klebsiella pneumoniae 64, Candida albicans 69. The growth inhibition zones were within 19-24 mm.

Supporting Agency

  • The work was carried out within the framework of the research work of the National University of Pharmacy “Pharmacognostic studies of the medicinal plant raw material and the development of phytotherapeutic agents based on it” (the state registration number 0114U000946).

Downloads

Download data is not yet available.

References

  1. Volianskyi, Yu. L.; Hrytsenko I. S.; Shyrobokov V. P. Vyvchennya spetsyfichnoyi aktyvnosti protymikrobnykh likarsʹkykh zasobiv. Metodychni rekomendatsiyi MOZ Ukrayiny [Study of the specific activity of antimicrobial drugs. Methodological recommendations of the Ministry of Health of Ukraine, in Ukrainian]; Zdorovja: Kyiv, 2004; 38 p.
  2. Derzhavna farmakopeia Ukrainy, 1 vydannia, 4 dopovnennya [The State Pharmacopoeia of Ukraine, 1st ed., 4th amendment, in Ukrainian]; State Enterprise “Ukrainian Scientific Pharmacopoeial Center for Quality of Medicines”: Kharkiv, 2011.
  3. Manso, T.; Lores, M.; De Miguel, T. Antimicrobial Activity of Polyphenols and Natural Polyphenolic Extracts on Clinical Isolates. Antibiotics 2021, 11 (1), 46. https://doi.org/10.3390/antibiotics11010046.
    | |
  4. Sherstiuk, M. The Analysis of Vitality Structure of Chimaphila Umbellata (L.) W. Barton CENOpopulations in Forest Phytocenoses of the Novgorod-Sivers’k Polissia. ScienceRise: Biological Science 2017, 0 (1 (4)), 40–45. https://doi.org/10.15587/2519-8025.2017.94019.
  5. Galván, I. J.; Mir-Rashed, N.; Jessulat, M.; Atanya, M.; Golshani, A.; Durst, T.; Petit, P.; Amiguet, V. T.; Boekhout, T.; Summerbell, R.; Cruz, I.; Arnason, J. T.; Smith, M. L. Antifungal and Antioxidant Activities of the Phytomedicine Pipsissewa, Chimaphila Umbellata. Phytochemistry 2008, 69 (3), 738–746. https://doi.org/10.1016/j.phytochem.2007.09.007.
    | |
  6. Raja, S; Ravindranadh, K; Keerthi, T. A Complete Profile on Chimaphila Umbellata – Traditional Uses, Pharmacological Activites and Phytoconstituents. ijp 2014, 6 (4), 464–470.
  7. Kovregin, O.; Prokopiuk, V.; Lytkin, D.; Vladymyrova, I. Study of the Influence of the Extract of Pipsissewa on Cell Cultures. ScienceRise: Pharmaceutical Science 2024, 3, 78–85. https://doi.org/10.15587/2519-4852.2024.307291.
  8. Ali, U.; Khan, M. M.; Khan, N.; Haya, R. T.; Asghar, M. U.; Abbasi, B. H. Chimaphila Umbellata; a Biotechnological Perspective on the Coming-of-Age Prince’s Pine. Phytochem. Rev. 2024, 23 (1), 229–244. https://doi.org/10.1007/s11101-023-09880-1.
    | |
  9. Yu, Y.; Elshafei, A.; Zheng, X.; Cheng, S.; Wang, Y.; Piao, M.; Wang, Y.; Jin, M.; Li, G.; Zheng, M. Chemical Constituents of Chimaphila Japonica Miq. Biochemical Systematics and Ecology 2021, 95, 104219. https://doi.org/10.1016/j.bse.2020.104219.
    |
  10. Marchenko, A.; Maslov, O.; Komisarenko, M.; Ponomarenko, S.; Osolodchenko, T.; Kolisnyk, S. Investigation Relationship between Antimicrobial, Antioxidant Activity and Biologically Active Compounds of Blackberry (Rubus Fruticocus L.) Fresh Fruit Liquid Extracts. AMI 2024, 4, 3–10. https://doi.org/10.5281/zenodo.14275647.
  11. Mogana, R.; Adhikari, A.; Tzar, M. N.; Ramliza, R.; Wiart, C. Antibacterial Activities of the Extracts, Fractions and Isolated Compounds from Canarium Patentinervium Miq. against Bacterial Clinical Isolates. BMC Complement Med Ther 2020, 20 (1), 55. https://doi.org/10.1186/s12906-020-2837-5.
    | |
  12. Zupanets, I. A.; Bezugla, N. P.; Otrishko, I. A.; Ursol, G. M. Modern medicines for herbal medicine: the view of a doctor and a pharmacist. Ukrainian medical journal 2023, 158 (6), 3-8. https://doi.org/10.32471/umj.1680-3051.158.249269.
  13. Nakaz Ministerstva okhorony zdorovʺya Ukrayiny vid 05.04.2007 r. No. 167 “Pro zatverdzhennya metodychnykh vkazivok “Vyznachennya chutlyvosti mikroorhanizmiv do antybakterialʹnykh preparative” [Order of the Ministry of Health of Ukraine dated 05.04.2007 No. 167 “On approval of methodological guidelines “Determination of the sensitivity of microorganisms to antibacterial drugs”, in Ukrainian]. https://zakon.rada.gov.ua/rada/show/v0167282-07#Text

Downloads

Published

2024-12-10

How to Cite

(1)
Kovregin, O. V.; Osolodchenko, T. P.; Vladymyrova, I. M. The Study of the Antibacterial Effect of the Umbellate Wintergreen Extract. J. Org. Pharm. Chem. 2024, 22, 43-47.

Issue

Vol. 22 No. 4 (2024): Issue in Progress

Section

Original Researches

License

Copyright (c) 2025 National University of Pharmacy

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors publishing their works in the Journal of Organic and Pharmaceutical Chemistry agree with the following terms:

1. Authors retain copyright and grant the journal the right of the first publication of the work under Creative Commons Attribution License allowing everyone to distribute and re-use the published material if proper citation of the original publication is given.

2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal’s published version of the work (e.g., post it to an institutional repository or publish it in a book) providing proper citation of the original publication.

3. Authors are permitted and encouraged to post their work online (e.g. in institutional repositories or on authors’ personal websites) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (see The Effect of Open Access).