Development of the methods for identification and quantitative determination of Epifine – a new perspective anticonvulsant

Authors

  • L. O. Perekhoda National University of Pharmacy, Ukraine

DOI:

https://doi.org/10.24959/ophcj.15.840

Keywords:

pharmaceutical analysis, identification, assay, anticonvulsant

Abstract

According to the results of the pharmacological testing the substance of 4-methoxy-3-chloroanilide 1-(3’-fluorophenyl)-5-methyl-1,2,3-triazole (1H)-4-carboxylic acid has shown a pronounced anticonvulsant activity that exceeds the level of the reference drug – Depakine. It is recommended for further profound studies under conditional name Epifine. Development of methods for the quality control of a substance occupies an important place among the stages of drug introduction.For preparation of the substance to introduction we have developed the methods for identification and quantitative determination of the promising substance Epifine. Standardization has been performed in accordance with the existing requirements for development of modern methods for analysis of pharmaceutical substances possessing the pharmacopoeial quality. For identification a set of physical, physicochemical (NMR 1H, IR and UV spectroscopy) and chemical methods has been proposed. The physical and chemical properties of Epifine have been investigated, and the intervals of melting point and solubility in different solvents have been determined. As expected, in the IR spectrum the characteristic absorption bands confirming the presence of aromatic rings, in particular benzene and triazole, carbonyl groups (amide-1, amide-2), substituted amino group, methyl and methoxy groups, were observed. We have also proposed the chemical methods of identification of this compound conditioned by the presence of the anilide residue, the triazole ring, and covalently bound halogens. The non-aqueous acid-base titration has been developed for the quantitative analysis of Epifine.

Downloads

Download data is not yet available.

References

  1. Gluschenko А. V., Rybalchenko Т. L., Shtrigol S. Yu., Georgiyants V. А., Perekhoda L. О. Ukr. biopharmats. Jrn., 2010, No.3(8), рр.28-34.
  2. Georgiyants V. А., Gluschenko А. V., Perekhoda L. О., Zaremba О. V. Zaporizkii Medical Journal, 2007, No.3(42), pp.91-94.
  3. Lyapunov N. А., Solovyov А. S, Stetsiv V. V. Yezhеnеdеlnik Аptека (Pharmacy Weekly), 2012, No.826 (4).
  4. Breinmaier E. Structure Elucidation by NMR in Organic Chemistry, John Wiley @ Sons Ltd, Chichester, 2002, 258 p.
  5. Perekhoda L. О. Molecular modeling and purposeful synthesis of anticonvulsants among derivatives of five-membered di(three)azaheterocycles, Manuscript. The thesis for the Doctor degree in Pharmacy, speciality 15.00.02 – Pharmaceutical Chemistry and Pharmacognosy, National University of Pharmacy, Kharkiv, 2013, 330 p.
  6. Braun D. Spectroscopy of organic substances, М.: Mir, 1992, 300 р.
  7. Silverstein R. M. Spectrometric Identification of Organic Compounds – 6th ed., N.Y.: John Wiley & Sons, 2001, 196 p.
  8. State Pharmacopoeia of Ukraine. 1th ed., Kh. SOE Scientific Expert Pharmacopeial Centre, 2001, 556 р.
  9. State Pharmacopoeia of Ukraine. 1th ed., 2 revised, Kh. SOE Scientific Expert Pharmacopeial Centre, 2008, 620 р.

Downloads

Published

2015-06-10

How to Cite

(1)
Perekhoda, L. O. Development of the Methods for Identification and Quantitative Determination of Epifine – a New Perspective Anticonvulsant. J. Org. Pharm. Chem. 2015, 13, 75-79.

Issue

Vol. 13 No. 2(50) (2015)

Section

Original Researches

License

Copyright (c) 2015 National University of Pharmacy

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Authors publishing their works in the Journal of Organic and Pharmaceutical Chemistry agree with the following terms:

1. Authors retain copyright and grant the journal the right of the first publication of the work under Creative Commons Attribution License allowing everyone to distribute and re-use the published material if proper citation of the original publication is given.

2. Authors are able to enter into separate, additional contractual arrangements for the non-exclusive distribution of the journal’s published version of the work (e.g., post it to an institutional repository or publish it in a book) providing proper citation of the original publication.

3. Authors are permitted and encouraged to post their work online (e.g. in institutional repositories or on authors’ personal websites) prior to and during the submission process, as it can lead to productive exchanges, as well as earlier and greater citation of published work (see The Effect of Open Access).